tuj-1 mouse 1:300 (Covance)
90
Structured Review
Covance
tuj-1 mouse 1:300
Tuj 1 Mouse 1:300, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tuj-1 mouse 1:300/product/Covance
Average 90 stars, based on 1 article reviews
Tuj 1 Mouse 1:300, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tuj-1 mouse 1:300/product/Covance
Average 90 stars, based on 1 article reviews
tuj-1 mouse 1:300 - by Bioz Stars,
2026-02
90/100 stars
Images
1) Product Images from "Immunohistochemical characterization of human olfactory tissue"
Article Title: Immunohistochemical characterization of human olfactory tissue
Journal: The Laryngoscope
doi: 10.1002/lary.21856
Figure Legend Snippet: Antibody Details
Techniques Used: Concentration Assay, Purification, Marker, Sequencing
Figure Legend Snippet: Non-neuronal components of the OE can be identified with various antibodies. A. DAB staining with the antibody to the transcription factor Hes1 labels nuclei of the supporting cells. B. The antibody to transcription factor Sox9 labels duct/gland cell nuclei only. C. Antibodies to K18 label both sustentacular cells as well duct/gland cells. D. Antibodies to E-Cadherin stain with a similar pattern to K18. E,F,G. Double immunofluorescent labeling of a sharp transitional zone between olfactory and respiratory epithelium with antibodies to Beta-tubulin IV and PGP9.5. Beta-tubulin IV densely labels the apical portion and cilia of the respiratory cells. Within the OE, PGP9.5 labels the cell body, axons and dendrites of the olfactory neurons. Several ciliated cells within the respiratory epithelium are labeled with both antibodies (arrows) and are clearly non-neuronal. Bg = bowman’s glands, arrowheads = basement membrane, scalebar = 25µ.
Techniques Used: Staining, Labeling
Figure Legend Snippet: Multiple antibodies can be used to identify subpopulations of olfactory neurons (ONs). A. DAB staining with OMP antibodies label mature ONs that tend to reside in the more apical portion of the epithelium. B. Antibodies to Tuj-1 label both mature and immature ONs with dense labeling of the dendrites and axons. C. The antibody to Golf labels mature and immature ONs with at a higher density in the dendrites and cell bodies. Arrows = dendritic knobs. D,E,F. Double immunofluorscent labeling of the epithelium with antibodies to PGP9.5 and OMP demonstrates the relative numbers of mature vs. immature ONs. Normal olfactory epithelium contains abundant OMP(+)/PGP9.5(+) mature neurons and OMP(−)/PGP9.5(+) immature neurons (arrows) suggesting a dynamic neuroepithelium. G,H,I. With double immunofluorescent labeling of the epithelium using HBC marker K5 and ON marker PGP9.5, cells are identified just above the HBCs that are absent of staining for either antibody (arrows). These cells may be GBCs or early neuroprogenitor cells. Asterisk identifies olfactory nerve fasicles extending out from the basal layer of the epithelium. Arrowheads = basement membrane, scale bar = 25µ.
Techniques Used: Staining, Labeling, Marker
Figure Legend Snippet: Transcription factor antibodies allow us to identify a subset of globose basal cells (GBCs). Immunofluorescent staining with Mash1 antibodies label clusters of cells in the OE (B,E,H,K). A,B,C. Mash1(+) cells (arrows) are not neurons as shown by lack of co-labeling with the anti-neuronal antibody Tuj-1. D,E,F. Mash1(+) cells are not HBCs as shown by absence of double staining with p63 antibodies. An arrow identifies a Mash1 stained cell sitting just above a p63 stained HBCs in a position typically occupied by GBCs. G,H,I. The absence of p27 double labeling with Mash1 (arrows) suggests that these cells are not quiescent. J,K,L. Many Mash1(+) cells (arrows) are mitotically active as shown by double labeling with Ki67 antibodies. M,N,O. Notch1 antibodies also label cells within the GBC compartment (arrows). Arrowheads = basement membrane, scale bar = 25µ.
Techniques Used: Staining, Labeling, Double Staining
Figure Legend Snippet: Staining Characteristics by Cell Type
Techniques Used: Staining, Activity Assay
Figure Legend Snippet: Esthesioneuroblastoma can be characterized using antibody reagents similar to that used for OE. A. DAB stained section of a portion of an esthesioneuroblastoma taken from a 44 year-old female using the neuron-specific antibody Tuj-1 labels the majority of the tumor as expected. B. However, these cells are not mature ONs based on the absence of OMP staining. C. The sustentacular and gland/duct cell specific K18 antibody labels cords of cell separating the tumor into nests. (Scale bar = 250µ for A, B, and C.) D. The tumor does not stain with antibodies to K5, p63 (E), and Beta-Tubulin IV (F). Only cells of the epithelial surface overlying the tumor stain with antibodies to K5, p63, and Beta-Tubulin IV indicating a respiratory-like epithelium. (Scale bar = 50µ for D, E, and F.) G. DAB staining using the Golf antibody also labels a large portion of the tumor in a pattern similar to Tuj-1 supporting an olfactory epithelial origin of the tumor. An arrow indicates extension of the tumor cells into the overlying epithelium (scale bar = 50µ). H. Immunofluorescent staining using antibodies to Mash1 also stains a large portion of the tumor in a pattern similar to Tuj-1 and Golf and corroborates previous mRNA findings. Arrows indicate areas of tumor extension into the overlying epithelium (scale bar = 100µ). I. DAB staining with Pax6 antibodies labels a large portion of the tumor and the overlying epithelium (scale bar = 50µ). Arrowheads = basement membrane.
Techniques Used: Staining
Figure Legend Snippet: Sox9(+)/Tuj-1(+) tumor cells do not follow the usual pattern of staining of olfactory epithelial gland cells. A,B,C. The separation of the tumor into two populations is confirmed using K18 and Tuj-1 antibodies. Similar to the two cell populations demonstrated with Sox2 and Sox9 antibodies, immunofluorescent labeling of cell cytoplasm and processes using K18 (arrows), does not co-label with the neuron-specific Tuj-1 antibody. D,E,F. E-Cad has a similar staining pattern as K18 in the epithelium labeling both gland and supporting cells, however within the tumor K18 is restricted to the Sox2(+)/Sox9(−)/Tuj-1(−) population while E-Cad labels the same NCAM(−) non-neuronal population (arrows) as well as the NCAM(+) neuronal population. Scale bar = 25µ.
Techniques Used: Staining, Labeling
Figure Legend Snippet: The esthesioneuroblastoma can be divided into two distinct populations using a combination of transcription factor and structural antibodies. A,B,C. Double immunofluorescent staining with Sox2 (arrows) and Sox9 antibodies show a mutually exclusive staining pattern within the tumor. Sox2(+) nuclei seem to occur at the periphery of the Sox9(+) cells and within the overlying epithelium (scale bar = 25µ). D,E,F. The staining of the tumor with Sox9 and K18 is also mutually exclusive and reveals a similar pattern to Sox9/Sox2 double staining (scale bar = 50µ). G,H,I. The Sox9 antibody labels Bowman’s duct/gland cell nuclei of the OE, but within the tumor it co-labels with Tuj-1(+) cells. The arrow indicates a neuronal process. Scale bar = 25µ.
Techniques Used: Staining, Double Staining
Figure Legend Snippet: A. The antibody to K17 labels only a portion of the tumor as shown with immunofluorescent staining and DAB at a lower magnification in the inset (inset scale bar = 100µ). B,C. Using double immunofluorescent staining with Tuj-1 a small number of cells are co-labeled with both antibodies (arrowheads), but most K17(+) cells seem to follow a pattern of staining similar to K18/Sox2 antibodies. White scale bar = 50µ.
Techniques Used: Staining, Labeling